Etheostoma zonale was collected from the Hocking River in south-central Ohio from April 1999 through August 2001. Ovaries of adult females were classified by developmental stage and appropriate-stage ovaries were used to analyze clutch size, egg size and gonadosomatic index (GSI). Although body condition was significantly low in 1999, we saw no direct evidence for a correlation with any reproductive trait in that year. Clutch size varied among years, though not in parallel with condition. Etheostoma zonale produced larger clutches and had larger GSI toward the middle of the breeding season in each year. Egg size declined over the season in each year. The amount of intraseasonal variation in reproductive traits here was similar to that previously reported for interannual and geographic variation in E. zonale and should caution investigators from generalizing from limited collections during the breeding season.